Developmental changes in the activity of messenger RNA isolated from germinating castor bean endosperm.

Mitochondria in the endosperm of the germinating castor bean; a developmental study.

Temperature-induced Phase Changes in the Membranes of Glyoxysomes, Mitochondria, and Proplastids from Germinating Castor Bean Endosperm.

Sucrose-gradient purified mitochondria, glyoxysomes, and proplastids from germinating castor bean (Ricinus communis L.) endosperm were examined by electron-spin resonance spectroscopy. A temperature-induced phase change was demonstrated in all of these organelles, their derivative membranes, and in micelles formed from the membrane phospholipids. The apparent transition temperature of the membr...

Accumulation of free ricinoleic Acid in germinating castor bean endosperm.

Lipids from the endosperm of germinating castor bean (Ricinus communis var. Hale) were separated by thin layer chromatography and quantitated by gas chromatography. During the later stages of lipid breakdown (4-6 days germination at 30 C), several lipid classes were found in addition to the storage triglycerides, which are triricinoleins for the most part. One was identified as free ricinoleic ...

ATP Citrate Lyase from Germinating Castor Bean Endosperm: Localization and some Properties.

ATP citrate lyase (EC 4.1.3.8) has been found in crude extracts from endosperm tissue of germinating castor bean and shows its maximum activity in 4- to 5-day-old seedlings. A strict requirement for coenzyme A and adenosine 5'-triphosphate was demonstrated. The pH optimum for the reaction is around 7.5. The unstable enzyme can be stabilized by freezing and addition of citrate and glycerol. (-)-...

Occurrence of RNA in glyoxysomes from castor bean endosperm.

The finding that in the endosperm of germinating castor bean, the enzymes of the glyoxylate cycle were housed in a special organelle, the glyoxysome (1, 2) raised the question of its biogenesis. We have shown that glyoxysome protein increases strikingly during the early stages of germination and concluded that rapid formation of new organelles occurs at this time (8). When the individual organe...